from typing import Annotated
import typer
from wasp2.cli import create_version_callback, verbosity_callback
from .run_counting import run_count_variants
from .run_counting_sc import run_count_variants_sc
def _get_counting_deps() -> dict[str, str]:
"""Get counting-specific dependency versions."""
import polars
import pysam
return {"polars": polars.__version__, "pysam": pysam.__version__}
_version_callback = create_version_callback(_get_counting_deps)
app = typer.Typer(
pretty_exceptions_short=False,
rich_markup_mode="rich",
help="[bold]WASP2 Counting[/bold] - Count alleles at variant positions in BAM files.",
epilog="[dim]Example: wasp2-count sample.bam variants.vcf.gz -o counts.tsv[/dim]",
)
[docs]
@app.callback(invoke_without_command=True)
def main(
ctx: typer.Context,
version: Annotated[
bool,
typer.Option(
"--version",
"-V",
callback=_version_callback,
is_eager=True,
help="Show version and dependency information.",
),
] = False,
verbose: Annotated[
bool,
typer.Option("--verbose", "-v", help="Enable verbose output with detailed progress."),
] = False,
quiet: Annotated[
bool,
typer.Option("--quiet", "-q", help="Suppress all output except errors."),
] = False,
) -> None:
"""WASP2 allele counting commands."""
verbosity_callback(verbose, quiet)
[docs]
@app.command()
def count_variants(
bam: Annotated[str, typer.Argument(help="BAM file")],
variants: Annotated[str, typer.Argument(help="Variant file (VCF, VCF.GZ, BCF, or PGEN)")],
samples: Annotated[
list[str] | None,
typer.Option(
"--samples",
"--sample",
"--samps",
"-s",
help=(
"One or more samples to use in variant file. "
"Accepts comma delimited string "
"or file with one sample per line"
),
),
] = None,
region_file: Annotated[
str | None,
typer.Option(
"--region",
"--regions",
"--region_file",
"--regions_file",
"-r",
help=(
"Only use variants overlapping regions in file. "
"Accepts BED or MACS2 formatted .(narrow/broad)Peak files. "
),
),
] = None,
out_file: Annotated[
str | None,
typer.Option(
"--out_file",
"--outfile",
"--out",
"-o",
help=("Output file for counts. Defaults to counts.tsv"),
),
] = None,
temp_loc: Annotated[
str | None,
typer.Option(
"--temp_loc",
"--temp",
"-t",
help=(
"Directory for keeping intermediary files. "
"Defaults to removing intermediary files using temp directory"
),
),
] = None,
use_region_names: Annotated[
bool,
typer.Option(
"--use_region_names",
help=(
"Use region names instead of coordinates. "
"Names are denoted in fourth column of BED. "
"Ignored if no name column in file. "
"Defaults to using coordinates."
),
),
] = False,
gene_feature: Annotated[
str | None,
typer.Option(
"--gene_feature",
"--feature",
"--feat",
help=(
"Feature type in gtf/gff3 for counting intersecting SNPs. "
"Defaults to 'exon' for snp counting"
),
),
] = None,
gene_attribute: Annotated[
str | None,
typer.Option(
"--gene_attribute",
"--attribute",
"--attributes",
"--attrs",
"--attr",
help=(
"Attribute name from gtf/gff3 attribute column to use as ID. "
"Defaults to '<feature>_id' in gtf and 'ID' in gff3"
),
),
] = None,
gene_parent: Annotated[
str | None,
typer.Option(
"--gene_parent",
"--parent",
"--parent_feature",
"--parent_attribute",
help=(
"Parent attribute in gtf/gff3 for feature used in counting"
"Defaults to 'transcript_id' in gtf and 'Parent' in gff3"
),
),
] = None,
use_rust: Annotated[
bool,
typer.Option(
"--use-rust/--no-rust",
help=(
"Use Rust acceleration for BAM counting (requires wasp2_rust extension). "
"Defaults to True if extension is available."
),
),
] = True,
vcf_bed: Annotated[
str | None,
typer.Option("--vcf-bed", help="Optional precomputed VCF bed file to skip vcf_to_bed."),
] = None,
intersect_bed: Annotated[
str | None,
typer.Option(
"--intersect-bed",
help="Optional precomputed intersect bed file to skip bedtools intersect.",
),
] = None,
include_indels: Annotated[
bool,
typer.Option(
"--include-indels/--no-indels",
help=(
"Include indels in addition to SNPs for variant processing. Default is SNPs only."
),
),
] = False,
) -> None:
sample_str = samples[0] if samples else None
run_count_variants(
bam_file=bam,
variant_file=variants,
region_file=region_file,
samples=sample_str,
use_region_names=use_region_names,
out_file=out_file,
temp_loc=temp_loc,
gene_feature=gene_feature,
gene_attribute=gene_attribute,
gene_parent=gene_parent,
use_rust=use_rust,
precomputed_vcf_bed=vcf_bed,
precomputed_intersect=intersect_bed,
include_indels=include_indels,
)
[docs]
@app.command()
def count_variants_sc(
bam: Annotated[str, typer.Argument(help="BAM file")],
variants: Annotated[str, typer.Argument(help="Variant file (VCF, VCF.GZ, BCF, or PGEN)")],
barcodes: Annotated[str, typer.Argument(help="File with one barcode per line. Used as index")],
samples: Annotated[
list[str] | None,
typer.Option(
"--samples",
"--sample",
"--samps",
"-s",
help=(
"One or more samples to use in variant file. "
"Accepts comma delimited string "
"or file with one sample per line. "
"RECOMMENDED TO USE ONE SAMPLE AT A TIME."
),
),
] = None,
feature_file: Annotated[
str | None,
typer.Option(
"--feature",
"--features",
"--feat",
"-f",
"--region",
"--regions",
"-r",
help=(
"Features used in single-cell experiment. "
"Only use variants overlapping features in file. "
"Accepts BED or MACS2 formatted .(narrow/broad)Peak files. "
"TODO: Implement genes gtf/gff format"
),
),
] = None,
out_file: Annotated[
str | None,
typer.Option(
"--out_file",
"--outfile",
"--out",
"-o",
help=(
"Output file to write Anndata allele counts. "
"H5ad file format. "
"Defaults to allele_counts.h5ad"
),
),
] = None,
temp_loc: Annotated[
str | None,
typer.Option(
"--temp_loc",
"--temp",
"-t",
help=(
"Directory for keeping intermediary files. "
"Defaults to removing intermediary files using temp directory"
),
),
] = None,
) -> None:
sample_str = samples[0] if samples else None
run_count_variants_sc(
bam_file=bam,
variant_file=variants,
barcode_file=barcodes,
feature_file=feature_file,
samples=sample_str,
out_file=out_file,
temp_loc=temp_loc,
)
